I learned yesterday that it does. For the last couple years I have used a silhoutte target, with playing cards taped to the ocular cavity and solar plexis, as those are the areas to aim at in a gunfight. I usually shoot about 200 through the cards perfectly in 3 shot, 1.5 second presentation from the holster. I've gotten really good after about 15k rounds.
Everything's great until my pistol instructor shows up, and tells me to use a competition silhouette target for the first time. My groups went from playing card size to 6 inches, off center left about 5 inches. I couldn't believe my eyes. He told me that when I used the cards, I was target shooting, not defensive shooting. So now I had to focus on the whole silhouette instead of easily identifiable playing cards. After about 2 mags, I was back in normal groups in center.
I never realised what a difference the target can make, I'm still amazed.